Vijayan, VikramO'Shea, Erin2014-09-162012Vijayan, Vikram, and Erin K. O’Shea. 2012. Sequence Determinants of Circadian Gene Expression Phase in Cyanobacteria. Journal of Bacteriology 195, no. 4: 665–671.0021-91931098-5530http://nrs.harvard.edu/urn-3:HUL.InstRepos:12872190The cyanobacterium Synechococcus elongatus PCC 7942 exhibits global biphasic circadian oscillations in gene expression under constant-light conditions. Class I genes are maximally expressed in the subjective dusk, whereas class II genes are maximally expressed in the subjective dawn. Here, we identify sequence features that encode the phase of circadian gene expression. We find that, for multiple genes, an ∼70-nucleotide promoter fragment is sufficient to specify class I or II phase. We demonstrate that the gene expression phase can be changed by random mutagenesis and that a single-nucleotide substitution is sufficient to change the phase. Our study provides insight into how the gene expression phase is encoded in the cyanobacterial genome.en-USJournal Article2014-09-1610.1128/JB.02012-12