Choi, PeterMeyerson, Matthew2014-11-032014Choi, Peter S., and Matthew Meyerson. 2014. “Targeted genomic rearrangements using CRISPR/Cas technology.” Nature communications 5 (1): 3728. doi:10.1038/ncomms4728. http://dx.doi.org/10.1038/ncomms4728.2041-1723http://nrs.harvard.edu/urn-3:HUL.InstRepos:13347629Genomic rearrangements are frequently observed in cancer cells but have been difficult to generate in a highly specific manner for functional analysis. Here we report the application of CRISPR/Cas technology to successfully generate several types of chromosomal rearrangements implicated as driver events in lung cancer, including the CD74-ROS1 translocation event and the EML4-ALK and KIF5B-RET inversion events. Our results demonstrate that Cas9-induced DNA breaks promote efficient rearrangement between pairs of targeted loci, providing a highly tractable approach for the study of genomic rearrangements.en-USJournal Article2014-11-0310.1038/ncomms4728