Abbaspourrad, AlirezaZhang, HuidanTao, YeCui, NaiwenAsahara, HaruichiZhou, YingYue, DongxianKoehler, StephanUng, Lloyd W.Heyman, JohnRen, YukunZiblat, RoyChong, ShaorongWeitz, David2015-09-012015Abbaspourrad, A., H. Zhang, Y. Tao, N. Cui, H. Asahara, Y. Zhou, D. Yue, et al. 2015. “Label-free single-cell protein quantification using a drop-based mix-and-read system.” Scientific Reports 5 (1): 12756. doi:10.1038/srep12756. http://dx.doi.org/10.1038/srep12756.2045-2322http://nrs.harvard.edu/urn-3:HUL.InstRepos:21461256Quantitative protein analysis of single cells is rarely achieved due to technical difficulties of detecting minute amounts of proteins present in one cell. We develop a mix-and-read assay for drop-based label-free protein analysis of single cells. This high-throughput method quantifies absolute, rather than relative, amounts of proteins and does not involve antibody labeling or mass spectrometry.en-USJournal Article2015-09-0110.1038/srep12756