Koussa, Mounir AHalvorsen, KenWard, AndrewWong, Wesley2015-09-012014Koussa, Mounir A, Ken Halvorsen, Andrew Ward, and Wesley P Wong. 2014. “DNA Nanoswitches: A quantitative platform for gel-based biomolecular interaction analysis.” Nature methods 12 (2): 123-126. doi:10.1038/nmeth.3209. http://dx.doi.org/10.1038/nmeth.3209.1548-7091http://nrs.harvard.edu/urn-3:HUL.InstRepos:21462643We introduce a nanoscale experimental platform that enables kinetic and equilibrium measurements of a wide range of molecular interactions by expanding the functionality of gel electrophoresis. Programmable, self-assembled DNA nanoswitches serve both as templates for positioning molecules, and as sensitive, quantitative reporters of molecular association and dissociation. We demonstrate this low cost, versatile, “lab-on-a-molecule” system by characterizing 10 different interactions, including a complex 4-body interaction with 5 discernable states.en-USDNA OrigamiNanoswitchMultimolecular InteractionsBiomolecular Interaction AnalysisKineticsAffinityGel ElectrophoresisJournal Article2015-09-0110.1038/nmeth.3209