Chan, Lawrence WCLin, XihongYung, GodwinLui, ThomasChiu, Ya MingWang, FengfengTsui, Nancy BYCho, William CSYip, SPSiu, Parco M.Wong, SC CesarYung, Benjamin YM2015-08-032015Chan, L. W., X. Lin, G. Yung, T. Lui, Y. M. Chiu, F. Wang, N. B. Tsui, et al. 2015. “Novel structural co-expression analysis linking the NPM1-associated ribosomal biogenesis network to chronic myelogenous leukemia.” Scientific Reports 5 (1): 10973. doi:10.1038/srep10973. http://dx.doi.org/10.1038/srep10973.2045-2322http://nrs.harvard.edu/urn-3:HUL.InstRepos:17820658Co-expression analysis reveals useful dysregulation patterns of gene cooperativeness for understanding cancer biology and identifying new targets for treatment. We developed a structural strategy to identify co-expressed gene networks that are important for chronic myelogenous leukemia (CML). This strategy compared the distributions of expressional correlations between CML and normal states, and it identified a data-driven threshold to classify strongly co-expressed networks that had the best coherence with CML. Using this strategy, we found a transcriptome-wide reduction of co-expression connectivity in CML, reflecting potentially loosened molecular regulation. Conversely, when we focused on nucleophosmin 1 (NPM1) associated networks, NPM1 established more co-expression linkages with BCR-ABL pathways and ribosomal protein networks in CML than normal. This finding implicates a new role of NPM1 in conveying tumorigenic signals from the BCR-ABL oncoprotein to ribosome biogenesis, affecting cellular growth. Transcription factors may be regulators of the differential co-expression patterns between CML and normal.en-USJournal Article2015-08-0310.1038/srep10973