Hedley-Whyte, E.2018-05-231975Hedley-Whyte, E. T. 1975. “Distribution of [1,2-3H]cholesterol in Mouse Brain after Injection in the Suckling Period.” The Journal of Cell Biology 66 (2) (August 1): 333–350. doi:10.1083/jcb.66.2.333.0021-9525http://nrs.harvard.edu/urn-3:HUL.InstRepos:37045424Glutaraldehyde-carbohydrazide polymer (GACH) was used to embed olfactory tracts, trapezoid body, and sciatic nerves of 9-, 10-, and 49- day old mice 2 h, 24 h, and 6 wk (respectively) after the intraperitoneal administration of [1,2-3H]cholesterol. Greater than 94% of radioactive cholesterol was retained in the GACH-infiltrated brain 24 h or more after injection. The fine structural preservation of both central and peripheral nervous tissues was excellent. Quantitative analysis of electron microscope autoradiographs demonstrated that [1,2- 3H]cholesterol is limited to blood vessel walls and lumen within the central nervous system at 2 h and 6 wk postinjection, but neurons and neuropil also contain the labeled cholesterol. The thickest myelin sheaths in the adult mice appear to be uniformly labeled throughout their width. No relationship of the retained [1,2-3H]cholesterol to the node of Ranvier was found in the adult sciatic nerve.en-USJournal Article2017-03-2219752018-05-2310.1083/jcb.66.2.333