Tabish, Ali M.Poels, KatrienByun, Hyang-Minluyts, KatrienBaccarelli, AndreaMartens, JohanKerkhofs, StefSeys, SvenHoet, PeterGodderis, Lode2017-02-182017Tabish, Ali M., Katrien Poels, Hyang-Min Byun, Katrien luyts, Andrea A. Baccarelli, Johan Martens, Stef Kerkhofs, Sven Seys, Peter Hoet, and Lode Godderis. 2017. “Changes in DNA Methylation in Mouse Lungs after a Single Intra-Tracheal Administration of Nanomaterials.” PLoS ONE 12 (1): e0169886. doi:10.1371/journal.pone.0169886. http://dx.doi.org/10.1371/journal.pone.0169886.1932-6203http://nrs.harvard.edu/urn-3:HUL.InstRepos:30371121Aims This study aimed to investigate the effects of nanomaterial (NM) exposure on DNA methylation. Methods and Results: Intra-tracheal administration of NM: gold nanoparticles (AuNPs) of 5-, 60- and 250-nm diameter; single-walled carbon nanotubes (SWCNTs) and multi-walled carbon nanotubes (MWCNTs) at high dose of 2.5 mg/kg and low dose of 0.25 mg/kg for 48 h to BALB/c mice. Study showed deregulations in immune pathways in NM-induced toxicity in vivo. NM administration had the following DNA methylation effects: AuNP 60 nm induced CpG hypermethylation in Atm, Cdk and Gsr genes and hypomethylation in Gpx; Gsr and Trp53 showed changes in methylation between low- and high-dose AuNP, 60 and 250 nm respectively, and AuNP had size effects on methylation for Trp53. Conclusion: Epigenetics may be implicated in NM-induced disease pathways.en-USBiology and life sciencesCell biologyChromosome biologyChromatinChromatin modificationDNA methylationGeneticsEpigeneticsGene expressionDNADNA modificationBiochemistryNucleic acidsEngineering and TechnologyNanotechnologyCarbon NanotubesBiology and Life SciencesPhysical SciencesMaterials ScienceMaterials by AttributeNanomaterialsPhysiologyImmune PhysiologyCytokinesMedicine and Health SciencesImmunologyImmune SystemInnate Immune SystemDevelopmental BiologyMolecular DevelopmentNanoparticlesZoologyAnimal AnatomyVibrissaeAnimal PhysiologyAnatomyBody FluidsBloodHematologyChanges in DNA Methylation in Mouse Lungs after a Single Intra-Tracheal Administration of NanomaterialsJournal Article2017-02-1810.1371/journal.pone.0169886