Chen, Y. GraceKowtoniuk, Walter EugeneAgarwal, IshaShen, YinghuaLiu, David2016-06-282009Chen, Y Grace, Walter E Kowtoniuk, Isha Agarwal, Yinghua Shen, and David R Liu. 2009. LC/MS analysis of cellular RNA reveals NAD-linked RNA. Nature Chemical Biology 5, no. 12: 879–881. doi:10.1038/nchembio.235.1552-4450http://nrs.harvard.edu/urn-3:HUL.InstRepos:27409160We developed a general method to detect cellular small molecule-RNA conjugates that does not rely on the reactivity of the small molecule, revealing NAD-linked RNA in E. coli and S. venezuelae. Subsequent characterization shows NAD is a 5’ modification of RNA, cannot be installed in vitro through aberrant transcriptional initiation, is only found among smaller cellular RNAs, and is present at a surprisingly high abundance of ~3000 copies per cell.en-USJournal Article2016-06-2810.1038/nchembio.235