Zhao, HongyuShen, AoXiang, Yang K.Corey, David2016-04-012016Zhao, Hongyu, Ao Shen, Yang K. Xiang, and David P. Corey. 2016. “Three Recombinant Engineered Antibodies against Recombinant Tags with High Affinity and Specificity.” PLoS ONE 11 (3): e0150125. doi:10.1371/journal.pone.0150125. http://dx.doi.org/10.1371/journal.pone.0150125.1932-6203http://nrs.harvard.edu/urn-3:HUL.InstRepos:26318510We describe three recombinant engineered antibodies against three recombinant epitope tags, constructed with divalent binding arms to recognize divalent epitopes and so achieve high affinity and specificity. In two versions, an epitope is inserted in tandem into a protein of interest, and a homodimeric antibody is constructed by fusing a high-affinity epitope-binding domain to a human or mouse Fc domain. In a third, a heterodimeric antibody is constructed by fusing two different epitope-binding domains which target two different binding sites in GFP, to polarized Fc fragments. These antibody/epitope pairs have affinities in the low picomolar range and are useful tools for many antibody-based applications.en-USBiology and Life SciencesBiochemistryProteinsRecombinant ProteinsBiotechnologyBioengineeringSynthetic BioengineeringMacromolecular EngineeringProtein EngineeringEngineering and TechnologySynthetic BiologyLuminescent ProteinsGreen Fluorescent ProteinMolecular BiologyMolecular Biology TechniquesMolecular Probe TechniquesImmunoblottingCell BiologyCell PhysiologyCell FusionAntibody EngineeringPrecipitation TechniquesImmunoprecipitationMedicine and Health SciencesHealth CareJournal Article2016-04-0110.1371/journal.pone.0150125