dos Santos, GilbertoSchroeder, Andrew J.Goodman, Joshua L.Strelets, Victor B.Crosby, Madeline A.Thurmond, JimEmmert, David B.Gelbart, William Martin2015-05-042015dos Santos, Gilberto, Andrew J. Schroeder, Joshua L. Goodman, Victor B. Strelets, Madeline A. Crosby, Jim Thurmond, David B. Emmert, and William M. Gelbart. 2015. “FlyBase: introduction of the Drosophila melanogaster Release 6 reference genome assembly and large-scale migration of genome annotations.” Nucleic Acids Research 43 (Database issue): D690-D697. doi:10.1093/nar/gku1099. http://dx.doi.org/10.1093/nar/gku1099.0305-1048http://nrs.harvard.edu/urn-3:HUL.InstRepos:15034860Release 6, the latest reference genome assembly of the fruit fly Drosophila melanogaster, was released by the Berkeley Drosophila Genome Project in 2014; it replaces their previous Release 5 genome assembly, which had been the reference genome assembly for over 7 years. With the enormous amount of information now attached to the D. melanogaster genome in public repositories and individual laboratories, the replacement of the previous assembly by the new one is a major event requiring careful migration of annotations and genome-anchored data to the new, improved assembly. In this report, we describe the attributes of the new Release 6 reference genome assembly, the migration of FlyBase genome annotations to this new assembly, how genome features on this new assembly can be viewed in FlyBase (http://flybase.org) and how users can convert coordinates for their own data to the corresponding Release 6 coordinates.en-USJournal Article2015-05-0410.1093/nar/gku1099