Pasqualini, Francesco SilvioSheehy, Sean PaulAgarwal, AshutoshAratyn-Schaus, YvonneParker, Kevin Kit2015-05-042015Pasqualini, Francesco Silvio, Sean Paul Sheehy, Ashutosh Agarwal, Yvonne Aratyn-Schaus, and Kevin Kit Parker. 2015. “Structural Phenotyping of Stem Cell-Derived Cardiomyocytes.” Stem Cell Reports 4 (3): 340-347. doi:10.1016/j.stemcr.2015.01.020. http://dx.doi.org/10.1016/j.stemcr.2015.01.020.2213-6711http://nrs.harvard.edu/urn-3:HUL.InstRepos:15034872Summary Structural phenotyping based on classical image feature detection has been adopted to elucidate the molecular mechanisms behind genetically or pharmacologically induced changes in cell morphology. Here, we developed a set of 11 metrics to capture the increasing sarcomere organization that occurs intracellularly during striated muscle cell development. To test our metrics, we analyzed the localization of the contractile protein α-actinin in a variety of primary and stem-cell derived cardiomyocytes. Further, we combined these metrics with data mining algorithms to unbiasedly score the phenotypic maturity of human-induced pluripotent stem cell-derived cardiomyocytes.en-USJournal Article2015-05-0410.1016/j.stemcr.2015.01.020