Chapman, AlecHe, Hertz ZiSijia, LuJun, YoungLongzhi, TanFuchou, X TangSunney, Xie2016-02-122015Chapman, Alec R., Zi He, Sijia Lu, Jun Yong, Longzhi Tan, Fuchou Tang, and X. Sunney Xie. 2015. “Single Cell Transcriptome Amplification with MALBAC.” Edited by Jung Weon Lee. PLoS ONE 10 (3) (March 30): e0120889. doi:10.1371/journal.pone.0120889.1932-6203http://nrs.harvard.edu/urn-3:HUL.InstRepos:25290368Recently, Multiple Annealing and Looping-Based Amplification Cycles (MALBAC) has been developed for whole genome amplification of an individual cell, relying on quasilinear instead of exponential amplification to achieve high coverage. Here we adapt MALBAC for single-cell transcriptome amplification, which gives consistently high detection efficiency, accuracy and reproducibility. With this newly developed technique, we successfully amplified and sequenced single cells from 3 germ layers from mouse embryos in the early gastrulation stage, and examined the epithelial-mesenchymal transition (EMT) program among cells in the mesoderm layer on a single-cell level.en-USJournal Article2015-03-03Alec R. Chapman, Zi He, Sijia Lu, Jun Yong, Longzhi Tan, Fuchou Tang, X. Sunney Xie2016-02-1210.1371/journal.pone.0120889