Ouyang, MengxingHill, I.Lee, Jung HyunHur, Soojung Claire2017-04-062017Ouyang, Mengxing, Winfield Hill, Jung Hyun Lee, and Soojung Claire Hur. 2017. “Microscale Symmetrical Electroporator Array as a Versatile Molecular Delivery System.” Scientific Reports 7 (1): 44757. doi:10.1038/srep44757. http://dx.doi.org/10.1038/srep44757.http://nrs.harvard.edu/urn-3:HUL.InstRepos:32072166Successful developments of new therapeutic strategies often rely on the ability to deliver exogenous molecules into cytosol. We have developed a versatile on-chip vortex-assisted electroporation system, engineered to conduct sequential intracellular delivery of multiple molecules into various cell types at low voltage in a dosage-controlled manner. Micro-patterned planar electrodes permit substantial reduction in operational voltages and seamless integration with an existing microfluidic technology. Equipped with real-time process visualization functionality, the system enables on-chip optimization of electroporation parameters for cells with varying properties. Moreover, the system’s dosage control and multi-molecular delivery capabilities facilitate intracellular delivery of various molecules as a single agent or in combination and its utility in biological research has been demonstrated by conducting RNA interference assays. We envision the system to be a powerful tool, aiding a wide range of applications, requiring single-cell level co-administrations of multiple molecules with controlled dosages.en-USJournal Article2017-04-0610.1038/srep44757