Janetzko, JohnTrauger, SuniaLazarus, Michael B.Walker, Suzanne2017-04-062016Janetzko, John, Sunia A. Trauger, Michael B. Lazarus, and Suzanne Walker. 2016. “How the glycosyltransferase OGT catalyzes amide bond cleavage.” Nature chemical biology 12 (11): 899-901. doi:10.1038/nchembio.2173. http://dx.doi.org/10.1038/nchembio.2173.http://nrs.harvard.edu/urn-3:HUL.InstRepos:32072168The essential human enzyme O-GlcNAc transferase (OGT), known for modulating the functions of nuclear and cytoplasmic proteins through Ser/Thr glycosylation, was unexpectedly implicated in the proteolytic maturation of the cell cycle regulator host cell factor-1 (HCF-1). Here we show that HCF-1 cleavage occurs via glycosylation of a glutamate side chain followed by on-enzyme formation of an internal pyroglutamate, which undergoes spontaneous backbone hydrolysis.en-USJournal Article2017-04-0610.1038/nchembio.2173