Mohr, StephanieHu, YanhuiEwen-Campen, BenjaminHousden, BenjaminViswanatha, RaghuvirPerrimon, Norbert2022-07-222016-09Mohr, Stephanie, Yanhui Hu, Benjamin Ewen-Campen, Benjamin Housden, Raghuvir Viswanatha, Norbert Perrimon. "CRISPR Guide RNA Design for Research Applications." FEBS Journal 283, no. 17 (2016): 3232-3238. DOI: 10.1111/febs.137771742-464X1742-4658https://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37372803The rapid rise of CRISPR as a technology for genome engineering and related research applications has created a need for algorithms and associated online tools that facilitate design of on‐target and effective guide RNAs (gRNAs). Here, we review the state of the art in CRISPR gRNA design for research applications of the CRISPR‐Cas9 system, including knockout, activation, and inhibition. Notably, achieving good gRNA design is not solely dependent on innovations in CRISPR technology. Good design and design tools also rely on availability of high‐quality genome sequence and gene annotations, as well as on availability of accumulated data regarding off‐targets and effectiveness metrics.en-USResearch Subject Categories::TECHNOLOGY::Bioengineering::BioinformaticsResearch Subject Categories::TECHNOLOGY::Bioengineering::Genetic engineering including functional genomicsJournal Article2016-07-0820162022-07-2210.1111/febs.13777