Lou, ShanAdam, YoavWeinstein, EliWilliams, E.Williams, K.Parot, VicenteKavokine, N.Liberles, StephenMadisen, L.Zeng, H.Cohen, Adam2017-07-112016Lou, S., Y. Adam, E. N. Weinstein, E. Williams, K. Williams, V. Parot, N. Kavokine, et al. 2016. “Genetically Targeted All-Optical Electrophysiology with a Transgenic Cre-Dependent Optopatch Mouse.” Journal of Neuroscience 36 (43) (October 26): 11059–11073. doi:10.1523/jneurosci.1582-16.2016.0270-6474http://nrs.harvard.edu/urn-3:HUL.InstRepos:33373324Recent advances in optogenetics have enabled simultaneous optical perturbation and optical readout of membrane potential in diverse cell types. Here, we develop and characterize a Cre-dependent transgenic Optopatch2 mouse line that we call Floxopatch. The animals expressed a blue-shifted channelrhodopsin, CheRiff, and a near infrared Archaerhodopsin-derived voltage indicator, QuasAr2, via targeted knock-in at the rosa26 locus. In Optopatch-expressing animals, we tested for overall health, genetically targeted expression, and function of the optogenetic components. In offspring of Floxopatch mice crossed with a variety of Cre driver lines, we observed spontaneous and optically evoked activity in vitro in acute brain slices and in vivo in somatosensory ganglia. Cell-type-specific expression allowed classification and characterization of neuronal subtypes based ontheir firing patterns. The Floxopatch mouse line is a usefultool for fast and sensitive characterization of neural activity in genetically specified cell types in intact tissue.en-USoptogeneticsoptopatchtransgenic micevoltage imagingJournal Article2017-05-1120162017-07-1110.1523/JNEUROSCI.1582-16.2016