Tang, QinAbdelfattah, Nouran S.Blackburn, Jessica S.Moore, John C.Martinez, Sarah A.Moore, Finola E.Lobbardi, RiadhTenente, Inês M.Ignatius, Myron S.Berman, Jason N.Liwski, Robert S.Houvras, YarivLangenau, David2015-03-022014Tang, Q., N. S. Abdelfattah, J. S. Blackburn, J. C. Moore, S. A. Martinez, F. E. Moore, R. Lobbardi, et al. 2014. “Optimized cell transplantation using adult rag2 mutant zebrafish.” Nature methods 11 (8): 821-824. doi:10.1038/nmeth.3031. http://dx.doi.org/10.1038/nmeth.3031.1548-7091http://nrs.harvard.edu/urn-3:HUL.InstRepos:14065308Cell transplantation into adult zebrafish has lagged behind mouse due to the lack of immune compromised models. Here, we have created homozygous rag2E450fs mutant zebrafish that have reduced numbers of functional T and B cells but are viable and fecund. Mutant fish engraft zebrafish muscle, blood stem cells, and cancers. rag2E450fs mutant zebrafish are the first immune compromised zebrafish model that permits robust, long-term engraftment of multiple tissues and cancer.en-USJournal Article2015-03-0210.1038/nmeth.3031