Aldrich, Leslie N.Kuo, Szu-YuCastoreno, Adam B.Goel, GautamKuballa, PetricRees, Matthew G.Seashore-Ludlow, Brinton A.Cheah, Jaime H.Latorre, Isabel J.Schreiber, StuartShamji, Alykhan F.Xavier, Ramnik2015-06-022015Aldrich, L., S. Kuo, A. B. Castoreno, G. Goel, P. Kuballa, M. G. Rees, B. A. Seashore-Ludlow, et al. 2015. “Discovery of a Small-Molecule Probe for V-ATPase Function.” Journal of the American Chemical Society 137 (16): 5563-5568. doi:10.1021/jacs.5b02150. http://dx.doi.org/10.1021/jacs.5b02150.0002-7863http://nrs.harvard.edu/urn-3:HUL.InstRepos:16120856Lysosomes perform a critical cellular function as a site of degradation for diverse cargoes including proteins, organelles, and pathogens delivered through distinct pathways, and defects in lysosomal function have been implicated in a number of diseases. Recent studies have elucidated roles for the lysosome in the regulation of protein synthesis, metabolism, membrane integrity, and other processes involved in homeostasis. Complex small-molecule natural products have greatly contributed to the investigation of lysosomal function in cellular physiology. Here we report the discovery of a novel, small-molecule modulator of lysosomal acidification derived from diversity-oriented synthesis through high-content screening.en-USArticleJournal Article2015-06-0210.1021/jacs.5b02150