Han, Sangyoon J.Oak, YoubeanGroisman, AlexDanuser, Gaudenz2016-02-012015Han, Sangyoon J., Youbean Oak, Alex Groisman, and Gaudenz Danuser. 2015. “Traction microscopy to identify force modulation in sub-resolution adhesions.” Nature methods 12 (7): 653-656. doi:10.1038/nmeth.3430. http://dx.doi.org/10.1038/nmeth.3430.1548-7091http://nrs.harvard.edu/urn-3:HUL.InstRepos:24983853We present a reconstruction algorithm that resolves cellular tractions in diffraction-limited nascent adhesions (NAs). The enabling method is the introduction of sparsity regularization to the solution of the inverse problem, which suppresses noise without underestimating traction magnitude. We show that NAs transmit a distinguishable amount of traction and that NA maturation depends on traction growth rate. A software package implementing this numerical approach is provided.en-USJournal Article2016-02-0110.1038/nmeth.3430