Akuthota, PraveenCapron, KelseyWeller, Peter2017-04-242014Akuthota, Praveen, Kelsey Capron, and Peter F. Weller. 2014. Eosinophil purification from peripheral blood. Eosinophils: 13–20. doi:10.1007/978-1-4939-1016-8_2.1064-3745http://nrs.harvard.edu/urn-3:HUL.InstRepos:32306655Eosinophils are granulocytes integral to allergic inflammation and parasitic responses and comprise 1-4 % of the circulating leukocytes in human beings under normal conditions. Isolation of human eosinophils allows for ex vivo and in vitro experimentation, providing a valuable tool for the study of allergic mechanisms. Here, we describe a technique for the isolation of human eosinophils by negative selection from whole blood obtained by venipuncture.en-UShumanseosinophilsgranulocytescell separation/instrumentationcell separation/methodsgranulocytesJournal Article2017-04-2410.1007/978-1-4939-1016-8_2