Sadegh, CameronMacklis, Jeffrey2015-04-012014Sadegh, C. and Macklis, J. D. 2014. Established monolayer differentiation of mouse embryonic stem cells generates heterogeneous neocortical-like neurons stalled at a stage equivalent to midcorticogenesis. Journal of Comparative Neurology 522: 2691–2706. doi: 10.1002/cne.235760021-9967http://nrs.harvard.edu/urn-3:HUL.InstRepos:14351448By assessing multiple positive and negative markers of forebrain progenitors and neurons, the authors show that now-standard, partially directed neocortical differentiation of mouse embryonic stem cells generates neurons that most closely resemble neocortical projection neurons during mid-corticogenesis. These neurons are“stalled” in subtype-specific molecular refinements, as compared to primary, dissociated E15.5 neocortical neurons cultured under the same in vitro conditions.en-USDirected differentiationneocortexprojection neuronpallial progenitorscorticogenesisJournal Article2015-03-12Cameron Sadegh; Jeffrey D Macklis2015-04-0110.1002/cne.23576