Sohail, MashaalVakhrusheva, Olga ASul, JaePulit, SaraFrancioli, Laurentvan den Berg, Leonard HVeldink, Jan Hde Bakker, PaulBazykin, Georgii AKondrashov, Alexey SSunyaev, Shamil2022-09-222016-07-29Sohail, Mashaal, Olga A. Vakhrusheva, Jae Hoon Sul, Sara L. Pulit, Laurent C. Francioli, Leonard H. van den Berg, Jan H. Veldink, et al. 2017. “Negative Selection in Humans and Fruit Flies Involves Synergistic Epistasis.” Science 356 (6337) (May 4): 539–542. doi:10.1126/science.aah5238. http://dx.doi.org/10.1126/science.aah5238.0036-8075https://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37373256AbstractNegative selection against deleterious alleles produced by mutation is the most common form of natural selection, which strongly influences within-population variation and interspecific divergence. However, some fundamental properties of negative selection remain obscure. In particular, it is still not known whether deleterious alleles affect fitness independently, so that cumulative fitness loss depends exponentially on the number of deleterious alleles, or synergistically, so that each additional deleterious allele results in a larger decrease in relative fitness. Negative selection with synergistic epistasis must produce negative linkage disequilibrium between deleterious alleles, and therefore, underdispersed distribution of the number of deleterious alleles in the genome. Indeed, we detected underdispersion of the number of rare loss-of-function (LoF) alleles in eight independent datasets from modern human andDrosophila melanogasterpopulations. Thus, ongoing selection against deleterious alleles is characterized by synergistic epistasis, which can explain how human and fly populations persist despite very high genomic deleterious mutation rates.en-USJournal Article2017-04-0720172022-09-2210.1101/066407