Szalay, TamasGolovchenko, Jene2015-11-062015Szalay, Tamas, and Jene A Golovchenko. 2015. “De Novo Sequencing and Variant Calling with Nanopores Using PoreSeq.” Nature Biotechnology 33 (10) (September 9): 1087–1091. doi:10.1038/nbt.3360.1087-0156http://nrs.harvard.edu/urn-3:HUL.InstRepos:23508734The single-molecule accuracy of nanopore sequencing has been an area of rapid academic and commercial advancement, but remains challenging for the de novo analysis of genomes. We introduce here a novel algorithm for the error correction of nanopore data, utilizing statistical models of the physical system in order to obtain high accuracy de novo sequences at a range of coverage depths. We demonstrate the technique by sequencing M13 bacteriophage DNA to 99% accuracy at moderate coverage as well as its use in an assembly pipeline by sequencing E. coli and DNA at a range of coverages. We also show the algorithm’s ability to accurately classify sequence variants at far lower coverage than existing methods.en-USJournal Article2016-02-2610.1038/nbt.3360