Reiser, Michael L.Mosaheb, MunirLisk, ChristinaPlatt, AndrewWetzler, Lee M.2017-06-152017Reiser, Michael L., Munir M. Mosaheb, Christina Lisk, Andrew Platt, and Lee M. Wetzler. 2017. “The TLR2 Binding Neisserial Porin PorB Enhances Antigen Presenting Cell Trafficking and Cross-presentation.” Scientific Reports 7 (1): 736. doi:10.1038/s41598-017-00555-4. http://dx.doi.org/10.1038/s41598-017-00555-4.http://nrs.harvard.edu/urn-3:HUL.InstRepos:33029758TOLL-like receptor (TLR) ligands activate both innate and adaptive immune cells, while modulating the cellular immune response. The outer membrane protein (OMP) from Neisseria meninigitidis, PorB, is a naturally occurring TLR2 ligand and functions as an adjuvant. Here, we demonstrate that PorB increases the level of OVA in the endo-/lysosomal cellular compartment of BMDCs, increases antigen presenting cell (APC) trafficking to draining lymph nodes, and enhances antigen cross-presentation. PorB is capable of mounting an antigen specific T cell response by efficiently stimulating antigen cross-presentation in vivo and in vitro assessed by BMDC OT-I cocultivation assays. The enhanced antigen cross-presentation and the increased APC recruitment to secondary lymphoid tissues expand the scope of known adjuvant effects of PorB on the immune system. Our findings lead to a better understanding of how TLR-ligand based adjuvants can alter and modulate immune responses.en-USJournal Article2017-06-1510.1038/s41598-017-00555-4