Park, JungwonElmlund, HansEricus, PeterYuk, Jong MinLimmer, David T.Chen, QianKim, KwanpyoHan, Sang HoonWeitz, DavidZettl, A.Alivisatos, A. Paul2017-08-152015Park, J., H. Elmlund, P. Ercius, J. M. Yuk, D. T. Limmer, Q. Chen, K. Kim, et al. 2015. “3D Structure of Individual Nanocrystals in Solution by Electron Microscopy.” Science 349 (6245) (July 16): 290–295. doi:10.1126/science.aab1343.0036-8075http://nrs.harvard.edu/urn-3:HUL.InstRepos:33758685Understanding structural details of colloidal nanoparticles is required to bridge our knowledge about their synthesis, growth mechanisms, and physical properties. We introduce a method for determining 3D structures of individual nanoparticles in solution. We combine a graphene liquid cell, high-resolution transmission electron microscopy, a direct electron detector, and an algorithm for single-particle 3D reconstruction originally developed for analysis of biological molecules to produce two near-atomic resolution 3D structures of individual Pt nanocrystals. Since our method derives the 3D structure from images of individual nanoparticles rotating freely in solution, it enables the analysis of heterogeneous populations of potentially unordered nanoparticles that are synthesized in solution, thereby providing a means to understand the structure and stability of defects at the nanoscale.en-USJournal Article2017-08-1510.1126/science.aab1343