Heianza, YorikoSun, DianjianyiMa, WenjieZheng, YanChampagne, Catherine M.Bray, George A.Sacks, FrankQi, Lu2022-06-142018-02-26Heianza, Yoriko, Dianjianyi Sun, Wenjie Ma, Yan Zheng, Catherine M. Champagne, George A. Bray, Frank Sacks et al. "Gut-Microbiome-Related LCT Genotype and 2-Year Changes in Body Composition and Fat Distribution: The POUNDS Lost Trial." International Journal of Obesity 42, no. 9 (2018): 1565-1573. DOI: 10.1038/s41366-018-0046-90307-05651476-5497https://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37372377Background/Objectives Gut microbiome regulates host energy metabolism and adiposity. A recent study identified a genome-wide significant variant in the lactase (LCT) gene that determines gut microbiome abundance. We investigated whether the LCT variant influenced long-term changes in adiposity among overweight and obese individuals. Subjects/Methods We included 583 whites with LCT variant rs4988235 (G allele as Bifidobacterium-abundance-increasing allele) who were randomly assigned to 1 of 4 weight-loss diets varying in macronutrient contents. Two-year changes in adiposity measures were assessed according to the LCT genotype and weight-loss diets. Results We observed a significant interaction between the LCT genotype and dietary protein intake on changes in whole body total fat mass %, trunk fat %, superficial adipose tissue mass (SAT), visceral adipose tissue mass (VAT), and total adipose tissue mass (TAT) (Pinteraction <0.05 for all). In response to high-protein diet, carrying the G allele of LCT variant rs4988235 was associated with greater reduction of whole body total fat mass % (β [SE] –0.9 [0.43], p=0.04), trunk fat % (–1.06 [0.58], p=0.07), SAT (–0.89 [0.42], p=0.04), VAT (–0.63 [0.27], p=0.03), and TAT (–1.69 [0.76], p=0.03). Conversely, increasing numbers of the G allele tended to be related to less reduction of these outcomes in response to low-protein diet. Conclusions Long-term improvement of body fat composition and distribution was significantly influenced by the Bifidobacterium-related LCT genotype and dietary protein intake. Overweight and obese individuals with the G allele of LCT variant rs4988235 may benefit improving adiposity by eating a low-calorie, high-protein diet.en-USResearch Subject Categories::MEDICINE::Physiology and pharmacology::Physiology::NutritionResearch Subject Categories::MEDICINE::Physiology and pharmacology::PhysiologyJournal Article2018-02-1620182022-06-1410.1038/s41366-018-0046-9