Wang, AnnaGarmann, ReesManoharan, Vinothan2023-01-242016-10-03Wang, Anna, Rees Garmann, Vinothan Manoharan. "Tracking E coli runs and tumbles with scattering solutions and digital holographic microscopy." Opt. Express 24, no. 21 (2016): 23719. DOI: 10.1364/oe.24.0237191094-4087https://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37374100We use in-line digital holographic microscopy to image freely swimming E. coli. We show that fitting a light scattering model to E. coli holograms can yield quantitative information about the bacterium’s body rotation and tumbles, offering a precise way to track fine details of bacterial motility. We are able to extract the cell’s three-dimensional (3D) position and orientation and recover behavior such as body angle rotation during runs, tumbles, and pole reversal. Our technique is label-free and capable of frame rates limited only by the camera.en-USAtomic and Molecular Physics, and OpticsJournal Article2018-01-2820162023-01-2410.1364/oe.24.023719