Hayashi, MasandoNinomiya, YoshifumiParsons, JaneyHayashi, KimikoOlsen, BjornTrelstad, Robert2022-07-221986-06-01Hayashi, Masando, Yoshifumi Ninomiya, Janey Parsons, Kimiko Hayashi, Bjorn Olsen, Robert Trelstad. "Differential Localization of mRNAs of Collagen Types I and II in Chick Fibroblasts, Chondrocytes, and Corneal Cells by In Situ Hybridization Using cDNA Probes." Journal of Cell Biology 102, no. 6 (1986): 2302-2309. DOI: 10.1083/jcb.102.6.23020021-95251540-8140https://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37372786We have employed a highly specific in situ hybridization protocol that allows differential detection of mRNAs of collagen types I and II in paraffin sections from chick embryo tissues. All probes were cDNA restriction fragments encoding portions of the C-propeptide region of the pro alpha-chain, and some of the fragments also encoded the 3'- untranslated region of mRNAs of either type I or type II collagen. Smears of tendon fibroblasts and those of sternal chondrocytes from 17- d-old chick embryos as well as paraffin sections of 10-d-old whole embryos and of the cornea of 6.5-d-old embryos were hybridized with 3H- labeled probes for either type I or type II collagen mRNA. Autoradiographs revealed that the labeling was prominent in tendon fibroblasts with the type I collagen probe and in sternal chondrocytes with the type II collagen probe; that in the cartilage of sclera and limbs from 10-d-old embryos, the type I probe showed strong labeling of fibroblast sheets surrounding the cartilage and of a few chondrocytes in the cartilage, whereas the type II probe labeled chondrocytes intensely and only a few fibroblasts; and that in the cornea of 6.5-d- old embryos, the type I probe labeled the epithelial cells and fibroblasts in the stroma heavily, and the endothelial cells slightly, whereas the type II probe labeled almost exclusively the epithelial cells except for a slight labeling in the endothelial cells. These data indicate that embryonic tissues express these two collagen genes separately and/or simultaneously and offer new approaches to the study of the cellular regulation of extracellular matrix components.en-USResearch Subject Categories::NATURAL SCIENCES::Biology::Cell and molecular biology::Cell biologyJournal Article2018-04-0719862022-07-2210.1083/jcb.102.6.2302