Kishida, YasuoOlsen, BjornBerg, RichardProckop, Darwin2022-07-191975-02-01Kishida, Yasuo, Bjorn Olsen, Richard Berg, Darwin Prockop. "Two Improved Methods for Preparing Ferritin-Protein Conjugates for Electron Microscopy." Journal of Cell Biology 64, no. 2 (1975): 331-339. DOI: 10.1083/jcb.64.2.3310021-95251540-8140https://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37372661Two improved procedures were developed for activating ferritin so that the ferritin could be covalently linked to antibodies. One procedure involved use of a water-soluble carbodiimide and N-hydroxysuccinimide to prepare ferritin-containing activated esters. The other involved activation of the ferritin with excess glutaraldehyde. The ferritin- antibody conjugates prepared with the two procedures were shown to have a number of properties which made them suitable for locating antigenic components in cells.en-USResearch Subject Categories::NATURAL SCIENCES::Biology::Cell and molecular biology::Cell biologyJournal Article2018-04-0719752022-07-1910.1083/jcb.64.2.331