Wolsk, Helene M.Chawes, Bo L.Litonjua, Augusto A.Hollis, Bruce W.Waage, JohannesStokholm, JakobBønnelykke, KlausBisgaard, HansWeiss, Scott2017-12-062017Wolsk, Helene M., Bo L. Chawes, Augusto A. Litonjua, Bruce W. Hollis, Johannes Waage, Jakob Stokholm, Klaus Bønnelykke, Hans Bisgaard, and Scott T. Weiss. 2017. “Prenatal vitamin D supplementation reduces risk of asthma/recurrent wheeze in early childhood: A combined analysis of two randomized controlled trials.” PLoS ONE 12 (10): e0186657. doi:10.1371/journal.pone.0186657. http://dx.doi.org/10.1371/journal.pone.0186657.http://nrs.harvard.edu/urn-3:HUL.InstRepos:34493290Background: We recently published two independent randomized controlled trials of vitamin D supplementation during pregnancy, both indicating a >20% reduced risk of asthma/recurrent wheeze in the offspring by 3 years of age. However, neither reached statistical significance. Objective: To perform a combined analysis of the two trials and investigate whether maternal 25-hydroxy-vitamin D (25(OH)D) level at trial entry modified the intervention effect. Methods: VDAART (N = 806) and COPSAC2010. (N = 581) randomized pregnant women to daily high-dose vitamin D3 (4,000 IU/d and 2,400 IU/d, respectively) or placebo. All women also received a prenatal vitamin containing 400 IU/d vitamin D3. The primary outcome was asthma/recurrent wheeze from 0-3yrs. Secondary end-points were specific IgE, total IgE, eczema and lower respiratory tract infections (LRTI). We conducted random effects combined analyses of the treatment effect, individual patient data (IPD) meta-analyses, and analyses stratified by 25(OH)D level at study entry. Results: The analysis showed a 25% reduced risk of asthma/recurrent wheeze at 0-3yrs: adjusted odds ratio (aOR) = 0.74 (95% CI, 0.57–0.96), p = 0.02. The effect was strongest among women with 25(OH)D level ≥30ng/ml at study entry: aOR = 0.54 (0.33–0.88), p = 0.01, whereas no significant effect was observed among women with 25(OH)D level <30ng/ml at study entry: aOR = 0.84 (0.62–1.15), p = 0.25. The IPD meta-analyses showed similar results. There was no effect on the secondary end-points. Conclusions: This combined analysis shows that vitamin D supplementation during pregnancy results in a significant reduced risk of asthma/recurrent wheeze in the offspring, especially among women with 25(OH)D level ≥ 30 ng/ml at randomization, where the risk was almost halved. Future studies should examine the possibility of raising 25(OH)D levels to at least 30 ng/ml early in pregnancy or using higher doses than used in our studies. Trial registration COPSAC2010: ClinicalTrials.gov NCT00856947; VDAART: ClinicalTrials.gov NCT00920621en-USPhysical sciencesChemistryChemical compoundsOrganic compoundsVitaminsVitamin DOrganic chemistryMedicine and Health SciencesWomen's HealthMaternal HealthPregnancyObstetrics and GynecologyMathematical and Statistical TechniquesStatistical MethodsMeta-AnalysisPhysical SciencesMathematicsStatistics (Mathematics)DermatologyEczemaPulmonologyAsthmaChemical CompoundsOrganic CompoundsOrganic ChemistryClinical MedicineClinical ImmunologyAllergiesAllergensBiology and Life SciencesImmunologyOrganismsEukaryotaAnimalsVertebratesAmniotesMammalsCatsJournal Article2017-12-0610.1371/journal.pone.0186657