Bhattacharyya, SanchariBershtein, ShimonShakhnovich, Eugene2017-12-062017Bhattacharyya, Sanchari, Shimon Bershtein, and Eugene I Shakhnovich. 2017. “Gene Dosage Experiments in Enterobacteriaceae Using Arabinose-regulated Promoters.” Bio-protocol 7 (14): e2396. doi:10.21769/BioProtoc.2396. http://dx.doi.org/10.21769/BioProtoc.2396.http://nrs.harvard.edu/urn-3:HUL.InstRepos:34493370This protocol is used to assay the effect of protein over-expression on fitness of E. coli. It is based on a plasmid expression of a protein of interest from an arabinose-regulated pBAD promoter followed by the measurement of the intracellular protein abundance by Western blot along with the measurement of growth parameters of E. coli cell expressing this protein.en-USGene-dosage toxicityArabinoseProtein abundanceWestern-blotFitnessOver-expressionPlasmidJournal Article2017-12-0610.21769/BioProtoc.2396