Schaus, ThomasWoo, SungwookXuan, FengChen, XiYin, Peng2017-12-052017Schaus, Thomas E., Sungwook Woo, Feng Xuan, Xi Chen, and Peng Yin. 2017. “A DNA nanoscope via auto-cycling proximity recording.” Nature Communications 8 (1): 696. doi:10.1038/s41467-017-00542-3. http://dx.doi.org/10.1038/s41467-017-00542-3.http://nrs.harvard.edu/urn-3:HUL.InstRepos:34491958Analysis of the spatial arrangement of molecular features enables the engineering of synthetic nanostructures and the understanding of natural ones. The ability to acquire a comprehensive set of pairwise proximities between components would satisfy an increasing interest in investigating individual macromolecules and their interactions, but current biochemical techniques detect only a single proximity partner per probe. Here, we present a biochemical DNA nanoscopy method that records nanostructure features in situ and in detail for later readout. Based on a conceptually novel auto-cycling proximity recording (APR) mechanism, it continuously and repeatedly produces proximity records of any nearby pairs of DNA-barcoded probes, at physiological temperature, without altering the probes themselves. We demonstrate the production of dozens of records per probe, decode the spatial arrangements of 7 unique probes in a homogeneous sample, and repeatedly sample the same probes in different states.en-USJournal Article2017-12-0510.1038/s41467-017-00542-3