Ye, GengtaiHuang, KunzhaiYu, JiangZhao, LiyingZhu, XianjunYang, QingbinLi, WendeJiang, YumingZhuang, BaoxiongLiu, HaoShen, ZhiyongWang, DaYan, LiZhang, LeiZhou, HaipengHu, YanfengDeng, HaijunLi, GuoxinQi, Xiaolong2017-12-052017Ye, G., K. Huang, J. Yu, L. Zhao, X. Zhu, Q. Yang, W. Li, et al. 2017. “MicroRNA-647 Targets SRF-MYH9 Axis to Suppress Invasion and Metastasis of Gastric Cancer.” Theranostics 7 (13): 3338-3353. doi:10.7150/thno.20512. http://dx.doi.org/10.7150/thno.20512.http://nrs.harvard.edu/urn-3:HUL.InstRepos:34491960MicroRNAs (miRNAs) play important roles in regulating tumour development and progression. Here we show that miR-647 is repressed in gastric cancer (GC), and associated with GC metastasis. Moreover, we identify that miR-647 can suppress GC cell migration and invasion in vitro. Mechanistically, we confirm miR-647 directly binds to the 3' untranslated regions of SRF mRNA, and SRF binds to the CArG box located at the MYH9 promoter. CCG-1423, an inhibitor of RhoA/SRF-mediated gene transcription, inhibits the expression of MYH9, especially in SRF downregulated cells. Overexpression of miR-647 inhibits MGC 80-3 cells' metastasis in orthotropic GC models, but increasing SRF expression in these cells reverses this change. Importantly, we found the synergistic inhibition effect of CCG-1423 and agomir-647, an engineered miRNA mimic, on cancer metastasis in orthotropic GC models. Our study demonstrates that miR-647 functions as a tumor metastasis suppressor in GC by targeting SRF/MYH9 axis.en-USGastric cancerMetastasisMicroRNA-647MYH9SRF.Journal Article2017-12-0510.7150/thno.20512