Han, DongranQi, XiaodongMyhrvold, CameronWang, BeiDai, MingjieJiang, ShuoxingBates, MaxwellLiu, YanAn, ByoungkwonZhang, FeiYan, HaoYin, Peng2021-09-132017-12-15Han, Dongran, Xiaodong Qi, Cameron Myhrvold, Bei Wang, Mingjie Dai, Shuoxing Jiang, Maxwell Bates et al. "Single-Stranded DNA and RNA Origami." Science 358, no. 6369 (2017): eaao2648. DOI: 10.1126/science.aao26480036-80751095-9203https://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37369406Self-folding of an information-carrying polymer into a defined structure is foundational to biology and offers attractive potential as a synthetic strategy. Although multicomponent self-assembly has produced complex synthetic nanostructures, unimolecular folding has seen limited progress. We describe a framework to design and synthesize a single DNA or RNA strand to self-fold into a complex yet unknotted structure that approximates an arbitrary user-prescribed shape. We experimentally construct diverse multikilobase single-stranded structures, including a ~10,000-nucleotide (nt) DNA structure and a ~6000-nt RNA structure. We demonstrate facile replication of the strand in vitro and in living cells. The work here thus establishes unimolecular folding as a general strategy for constructing complex and replicable nucleic acid nanostructures, and expands the design space and material scalability for bottom-up nanotechnology.en-USResearch Subject Categories::NATURAL SCIENCES::Biology::Cell and molecular biology::GeneticsJournal Article2017-10-0720172021-09-1310.1126/science.aao2648