Lee, GeonHuiLee, JuKyungKim, JeongHoonChoi, Hak SooKim, JonghanLee, SangHoonLee, HeaYeon2017-11-212017Lee, GeonHui, JuKyung Lee, JeongHoon Kim, Hak Soo Choi, Jonghan Kim, SangHoon Lee, and HeaYeon Lee. 2017. “Single Microfluidic Electrochemical Sensor System for Simultaneous Multi-Pulmonary Hypertension Biomarker Analyses.” Scientific Reports 7 (1): 7545. doi:10.1038/s41598-017-06144-9. http://dx.doi.org/10.1038/s41598-017-06144-9.http://nrs.harvard.edu/urn-3:HUL.InstRepos:34375125Miniaturized microfluidic biosensors have recently been advanced for portable point-of-care diagnostics by integrating lab-on-a-chip technology and electrochemical analysis. However, the design of a small, integrated, and reliable biosensor for multiple and simultaneous electrochemical analyses in a single device remains a challenge. Here, we present a simultaneous microfluidic electrochemical biosensing system to detect multiple biomarkers of pulmonary hypertension diseases in a single device. The miniaturized biosensor, which is composed of five chambers, is precisely and individually controlled using in-house-built pneumatic microvalves to manipulate the flow pathway. Each chamber is connected to an electrochemical sensor designed to detect four different biomarkers plus a reference control. Our design allows for loading of multiple reagents for simultaneous analyses. On the basis of the developed microfluidic electrochemical sensor system, we successfully detected four well-defined pulmonary hypertension-associated biomarkers, namely, fibrinogen, adiponectin, low-density lipoprotein, and 8-isoprostane. This novel approach offers a new platform for a rapid, miniaturized, and sensitive diagnostic sensor in a single device for various human diseases.en-USJournal Article2017-11-2110.1038/s41598-017-06144-9