Berneman, Zwi N.Ablashi, DharamLi, GeEger-Fletcher, MaureenReitz, Marvin S.Hung, Chia-LingBrus, IrenaKomaroff, AnthonyGallo, Robert C.2021-09-271992-11-01Berneman, Zwi N., Dharam Ablashi, Ge Li, Maureen Eger-Fletcher, Marvin S. Reitz, Chia-Ling Hung, Irena Brus et al. "Human Herpesvirus 7 is a T-Lymphotropic Virus and is Related to, but Significantly Different from, Human Herpesvirus 6 and Human Cytomegalovirus." Proceedings of the National Academy of Sciences 89, no. 21 (1992): 10552-10556. DOI: 10.1073/pnas.89.21.105520027-84241091-6490https://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37369593An independent strain (JI) of human herpesvirus 7 (HHV-7) was isolated from a patient with chronic fatigue syndrome (CFS). No significant association could be established by seroepidemiology between HHV-7 and CFS. HHV-7 is a T-lymphotropic virus, infecting CD4+ and CD8+ primary lymphocytes. HHV-7 can also infect SUP-T1, an immature T-cell line, with variable success. Southern blot analysis with DNA probes scanning 58.8% of the human herpesvirus 6 (HHV-6) genome and hybridizing to all HHV-6 strains tested so far revealed homology to HHV-7 with only 37.4% of the total probe length. HHV-7 contains the GGGTTA repetitive sequence, as do HHV-6 and Marek's disease chicken herpesvirus. DNA sequencing of a 186-base-pair fragment of HHV-7(JI) revealed an identity with HHV-6 and human cytomegalovirus of 57.5% and 36%, respectively. Oligonucleotide primers derived from this sequence (HV7/HV8, HV10/HV11) amplified HHV-7 DNA only and did not amplify DNA from other human herpesviruses, including 12 different HHV-6 strains. Southern blot analysis with the p43L3 probe containing the 186-base-pair HHV-7 DNA fragment hybridized to HHV-7 DNA only. The molecular divergence between human cytomegalovirus, on the one hand, and HHV-6 and HHV-7, on the other, is greater than between HHV-6 and HHV-7, which, in turn, is greater than the difference between HHV-6 strains. This study supports the classification of HHV-7 as an additional member of the human beta-herpesviruses.en-USMultidisciplinaryJournal Article2018-06-2419922021-09-2710.1073/pnas.89.21.10552