Jiang, NanYing, Guo-LiangYetisen, Ali K.Montelongo, YunuenShen, LingXiao, Yu-XuanBusscher, Henk J.Yang, Xiao-YuSu, Bao-Lian2018-07-252018Jiang, Nan, Guo-Liang Ying, Ali K. Yetisen, Yunuen Montelongo, Ling Shen, Yu-Xuan Xiao, Henk J. Busscher, Xiao-Yu Yang, and Bao-Lian Su. 2018. “A bilayered nanoshell for durable protection of single yeast cells against multiple, simultaneous hostile stimuli† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c8sc01130c.” Chemical Science 9 (21): 4730-4735. doi:10.1039/c8sc01130c. http://dx.doi.org/10.1039/c8sc01130c.http://nrs.harvard.edu/urn-3:HUL.InstRepos:37298545Single cell surface engineering provides the most efficient, non-genetic strategy to enhance cell stability. However, it remains a huge challenge to improve cell stability in complex artificial environments. Here, a soft biohybrid interfacial layer is fabricated on individual living-cell surfaces by their exposure to a suspension of gold nanoparticles and l-cysteine to form a protecting functional layer to which porous silica layers were bound yielding pores with a diameter of 3.9 nm. The living cells within the bilayered nanoshells maintained high viability (96 ± 2%) as demonstrated by agar plating, even after five cycles of simultaneous exposure to high temperature (40 °C), lyticase and UV light. Moreover, yeast cells encapsulated in bilayered nanoshells were more recyclable than native cells due to nutrient storage in the shell.en-USJournal Article2018-07-2510.1039/c8sc01130c