Kiani, SamiraChavez, AlejandroTuttle, MarcelleHall, Richard NChari, RajTer-Ovanesyan, DmitryQian, JasonPruitt, Benjamin WBeal, JacobVora, SuhaniBuchthal, JoannaKowal, Emma J KEbrahimkhani, Mohammad RCollins, JamesWeiss, RonChurch, George2022-07-222015-11Kiani, Samira, Alejandro Chavez, Marcelle Tuttle, Richard N Hall, Raj Chari, Dmitry Ter-Ovanesyan, Jason Qian et al. "Cas9 gRNA Engineering for Genome Editing, Activation and Repression." Nature Methods 12, no. 11 (2015): 1051-1054. DOI: 10.1038/nmeth.35801548-70911548-7105https://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37372787We demonstrate that by altering the length of Cas9-associated guide RNA(gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein.en-USResearch Subject Categories::NATURAL SCIENCES::Biology::Cell and molecular biology::GeneticsJournal Article2017-08-0220152022-07-2210.1038/nmeth.3580