Robasky, KimberlyLewis, Nathan EChurch, George2022-07-222014-01Robasky, Kimberly, Nathan E Lewis, George Church. "The Role of Replicates for Error Mitigation in Next-Generation Sequencing." Nature Reviews Genetics 15, no. 1 (2014): 56-62. DOI: 10.1038/nrg36551471-00561471-0064https://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37372790Advances in next-generation technologies have rapidly improved sequencing fidelity and significantly decreased sequencing error rates. However, with billions of nucleotides in a human genome, even low experimental error rates yield many errors in variant calls. Erroneous variants can mimic true somatic and rare variants, thus requiring costly confirmatory experiments to minimize the number of false positives. Here we discuss sources of experimental error in next-generation sequencing and how replicates can be used to abate them.en-USResearch Subject Categories::MEDICINE::Dermatology and venerology,clinical genetics, internal medicine::Clinical geneticsResearch Subject Categories::MEDICINE::Dermatology and venerology,clinical genetics, internal medicine::Clinical genetics::Molecular biologyJournal Article2017-08-0220142022-07-2210.1038/nrg3655