Balanced Diet-Fed Fat-1 Transgenic Mice Exhibit Lower Hindlimb Suspension-Induced Soleus Muscle Atrophy

Marzuca-Nassr, Gabriel NasriMurata, Gilson MasahiroMartins, Amanda RoqueVitzel, Kaio FernandoCrisma, Amanda RabelloTorres, Rosângela PavanMancini-Filho, JorgeKang, JingCuri, Rui2017-12-062017Marzuca-Nassr, Gabriel Nasri, Gilson Masahiro Murata, Amanda Roque Martins, Kaio Fernando Vitzel, Amanda Rabello Crisma, Rosângela Pavan Torres, Jorge Mancini-Filho, Jing Xuan Kang, and Rui Curi. 2017. “Balanced Diet-Fed Fat-1 Transgenic Mice Exhibit Lower Hindlimb Suspension-Induced Soleus Muscle Atrophy.” Nutrients 9 (10): 1100. doi:10.3390/nu9101100. http://dx.doi.org/10.3390/nu9101100.http://nrs.harvard.edu/urn-3:HUL.InstRepos:34492892The consequences of two-week hindlimb suspension (HS) on skeletal muscle atrophy were investigated in balanced diet-fed Fat-1 transgenic and C57BL/6 wild-type mice. Body composition and gastrocnemius fatty acid composition were measured. Skeletal muscle force, cross-sectional area (CSA), and signaling pathways associated with protein synthesis (protein kinase B, Akt; ribosomal protein S6, S6, eukaryotic translation initiation factor 4E-binding protein 1, 4EBP1; glycogen synthase kinase3-beta, GSK3-beta; and extracellular-signal-regulated kinases 1/2, ERK 1/2) and protein degradation (atrophy gene-1/muscle atrophy F-box, atrogin-1/MAFbx and muscle RING finger 1, MuRF1) were evaluated in the soleus muscle. HS decreased soleus muscle wet and dry weights (by 43% and 26%, respectively), muscle isotonic and tetanic force (by 29% and 18%, respectively), CSA of the soleus muscle (by 36%), and soleus muscle fibers (by 45%). Fat-1 transgenic mice had a decrease in the ω-6/ω-3 polyunsaturated fatty acids (PUFAs) ratio as compared with C57BL/6 wild-type mice (56%, p < 0.001). Fat-1 mice had lower soleus muscle dry mass loss (by 10%) and preserved absolute isotonic force (by 17%) and CSA of the soleus muscle (by 28%) after HS as compared with C57BL/6 wild-type mice. p-GSK3B/GSK3B ratio was increased (by 70%) and MuRF-1 content decreased (by 50%) in the soleus muscle of Fat-1 mice after HS. Balanced diet-fed Fat-1 mice are able to preserve in part the soleus muscle mass, absolute isotonic force and CSA of the soleus muscle in a disuse condition.en-USFat-1 micemuscle disuse atrophyω-3 PUFAshindlimb suspensionprotein synthesis/degradation signalingBalanced Diet-Fed Fat-1 Transgenic Mice Exhibit Lower Hindlimb Suspension-Induced Soleus Muscle AtrophyJournal Article2017-12-0610.3390/nu9101100