Ni, Jian-QuanMarkstein, MicheleBinari, RichardPfeiffer, Barret D.Liu, Lu-PingVillalta, ChristiansBooker, MatthewPerkins, LizabethPerrimon, Norbert2022-07-062008-01Ni, Jian-Quan, Michele Markstein, Richard Binari, Barret D. Pfeiffer, Lu-Ping Liu, Christians Villalta, Matthew Booker et al. "Vector and Parameters for Targeted Transgenic RNA Interference in Drosophila melanogaster." Nature Methods 5, no. 1 (2008): 49-51. DOI: 10.1038/nmeth11461548-70911548-7105https://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37372493The conditional expression of hairpin constructs in Drosophila melanogaster has emerged in recent years as a method of choice in functional genomic studies. To date, upstream activating site–driven RNA interference constructs have been inserted into the genome randomly using P-element–mediated transformation, which can result in false negatives due to variable expression. To avoid this problem, we have developed a transgenic RNA interference vector based on the phiC31 site-specific integration method.en-USResearch Subject Categories::NATURAL SCIENCES::Chemistry::Biochemistry::Functional genomicsJournal Article2022-07-0610.1038/nmeth1146