Hume, Maxwell A.Barrera, Luis A.Gisselbrecht, Stephen S.Bulyk, Martha L.2019-10-052015Hume, Maxwell A., Luis A. Barrera, Stephen S. Gisselbrecht, and Martha L. Bulyk. 2014. “UniPROBE, Update 2015: New Tools and Content for the Online Database of Protein-Binding Microarray Data on protein–DNA Interactions.” Nucleic Acids Research 43 (D1): D117–22. https://doi.org/10.1093/nar/gku1045.0305-10481362-4962http://nrs.harvard.edu/urn-3:HUL.InstRepos:41483027The Universal PBM Resource for Oligonucleotide Binding Evaluation (UniPROBE) serves as a convenient source of information on published data generated using universal protein-binding microarray (PBM) technology, which provides in vitro data about the relative DNA-binding preferences of transcription factors for all possible sequence variants of a length k ('k-mers'). The database displays important information about the proteins and displays their DNA-binding specificity data in terms of k-mers, position weight matrices and graphical sequence logos. This update to the database documents the growth of UniPROBE since the last update 4 years ago, and introduces a variety of new features and tools, including a new streamlined pipeline that facilitates data deposition by universal PBM data generators in the research community, a tool that generates putative nonbinding (i.e. negative control) DNA sequences for one or more proteins and novel motifs obtained by analyzing the PBM data using the BEEML-PBM algorithm for motif inference. The UniPROBE database is available at http://uniprobe.org.en-USJournal Article2019-10-0510.1093/nar/gku1045