Liu, GuoxiaPapa, ArianneKatchman, Alexander N.Zakharov, Sergey I.Roybal, DanielHennessey, JessicaKushner, JaredYang, LinChen, Bi-XingKushnir, AlexanderDangas, KaterinaGygi, StevenPitt, Geoffrey S.Colecraft, Henry M.Ben-Johny, ManuKalocsay, MarianMarx, Steven O.2023-02-162020-01-22Liu, Guoxia, Arianne Papa, Alexander N. Katchman, Sergey I. Zakharov, Daniel Roybal, Jessica A. Hennessey, Jared Kushner et al. 2020. "Mechanism of adrenergic Ca V 1.2 stimulation revealed by proximity proteomics." Nature 577, no. 7792: 695-700.0028-08361476-4687https://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37374373Increased cardiac contractility during fight-or-flight response is caused by b-adrenergic augmentation of CaV1.2 channels. In transgenic murine hearts expressing fully PKA phosphorylation-site-deficient mutant CaV1.2 a1C and b subunits, this regulation persists, implying involvement of extra-channel factors. Here, we identify the mechanism by which b-adrenergic agonists stimulate voltage-gated Ca2+ channels. We expressed ascorbate-peroxidase-conjugated-a1C or -b2B subunits in mouse hearts and used multiplexed, quantitative proteomics to track hundreds of proteins in proximity of CaV1.2. We observed that the Ca2+ channel inhibitor Rad, a monomeric G-protein, is enriched in the CaV1.2 micro-environment but is depleted during b-adrenergic stimulation. PKA-catalyzed phosphorylation of specific Ser residues on Rad decreases its affinity for auxiliary b-subunits and relieves constitutive inhibition of CaV1.2 observed as an increase in channel open probability. Expression of Rad or Rem, a homolog, also imparted PKA-induced stimulation of CaV1.3 and CaV2.2, revealing an evolutionarily conserved mechanism that confers adrenergic-modulation upon voltage-gated Ca2+ channels.en-USMultidisciplinaryJournal Article2023-02-1610.1038/s41586-020-1947-z