Recht, JudithKolter, Roberto2019-10-052001Recht, J., and R. Kolter. 2001. “Glycopeptidolipid Acetylation Affects Sliding Motility and Biofilm Formation in Mycobacterium Smegmatis.” Journal of Bacteriology 183 (19): 5718–24. https://doi.org/10.1128/jb.183.19.5718-5724.2001.0021-91931067-88321098-5530http://nrs.harvard.edu/urn-3:HUL.InstRepos:41483238The absence of glycopeptidolipids (GPLs) abolishes the ability of mycobacteria both to slide over the surface of motility plates and to form biofilms on polyvinyl chloride. In a screen for biofilm-defective mutants of Mycobacterium smegmatis mc(2) 155, a new mutant was obtained that resulted in partial inhibition of both processes and also showed an intermediate rough colony morphology. The mariner transposon insertion mapped to a GPL biosynthesis gene (atf1) which encodes a putative acetyltranferase involved in the transfer of acetyl groups to the glycopeptide core. Physical characterization of the GPLs from the atf1 mutant demonstrated that they were not acetylated.en-USJournal Article2019-10-0510.1128/JB.183.19.5718-5724.2001