Synthesis of an electronically-tuned minimally interfering alkynyl photo-affinity label to measure small molecule

Chang, Chia-fuMfuh, AdelpheGao, JinxuWu, Hung-YiWoo, Christina2020-04-062018-06Chang, Mfuh, Gao, Wu, and Woo. "Synthesis of an Electronically-tuned Minimally Interfering Alkynyl Photo-affinity Label to Measure Small Molecule–protein Interactions." Tetrahedron 74, no. 26 (2018): 3273-277.0040-4020http://nrs.harvard.edu/urn-3:HUL.InstRepos:42656537We report the synthesis of an electronically-tuned minimally interfering photo-affinity label (MI-PAL), a compact five-carbon tag functionalized with an alkyl diazirine and alkyne handle. MI-PAL is compatible with protein photo-conjugation, click chemistry and mass spectrometry and readily installed to complex molecules for biological target identification.en-USphotochemistrydiazirinephoto-affinity labelingchemical proteomicsCuAAmass spectrometrySynthesis of an electronically-tuned minimally interfering alkynyl photo-affinity label to measure small molecule–protein interactionsJournal Article2020-04-0610.1016/j.tet.2018.03.024