Lee, Jonathan D.Paulo, JoaoPosey, Ryan R.Mugoni, VeraKong, NikkiCheloni, GiuliaLee, Yu-RuSlack, FrankTenen, DanielClohessy, JohnGygi, StevenPandolfi, Pier Paolo2022-08-262021-03-01Lee, Jonathan D, Joao A Paulo, Ryan R Posey, Vera Mugoni, Nikki R Kong, Giulia Cheloni, Yu-Ru Lee, et al. 2021. “Dual DNA and Protein Tagging of Open Chromatin Unveils Dynamics of Epigenomic Landscapes in Leukemia.” Nature Methods 18 (3): 293–302.1548-70911548-7105https://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37373075The architecture of chromatin specifies eukaryotic cell identity by controlling transcription factor access to sites of gene regulation. Here we describe a dual transposase/peroxidase approach, integrative DNA And Protein Tagging (iDAPT), which detects both DNA (iDAPT-seq) and protein (iDAPT-MS) associated with accessible regions of chromatin. In addition to direct identification of bound transcription factors, iDAPT enables the inference of their gene regulatory networks, protein interactors, and regulation of chromatin accessibility. We applied iDAPT to profile the epigenomic consequences of granulocytic differentiation of acute promyelocytic leukemia, yielding previously undescribed mechanistic insights with potential therapeutic implications. Our findings demonstrate the power of iDAPT as a discovery platform for both the dynamic epigenomic landscapes and their transcription factor components associated with biological phenomena and disease.en-USCell BiologyMolecular BiologyBiochemistryBiotechnologyJournal Article2022-08-2610.1038/s41592-021-01077-8