Yan, DongNeumüller, Ralph A.Buckner, MichaelAyers, KathleenLi, HuaHu, YanhuiYang-Zhou, DonghuiPan, LeiWang, XiaoxiKelley, ColleenVinayagam, ArunachalamBinari, RichardRandklev, SakaraPerkins, Lizabeth A.Xie, TingCooley, LynnPerrimon, Norbert2021-06-252014-02-24Yan, Dong, Ralph A. Neumüller, Michael Buckner, Kathleen Ayers, Hua Li, Yanhui Hu, Donghui Yang-Zhou et al. "A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal." Developmental Cell 28, no. 4 (2014): 459-473. DOI: 10.1016/j.devcel.2014.01.0201534-58071878-1551https://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37368038Stem cells possess the capacity to generate two cells of distinct fate upon division; one cell retaining stem cell identity and the other cell destined to differentiate. These cell fates are established by cell-type-specific genetic networks. To comprehensively identify components of these networks, we performed a large-scale RNAi screen in Drosophila female germline stem cells (GSCs) covering ~25% of the genome. The screen identified 366 genes that affect GSC maintenance, differentiation or other processes involved in oogenesis. Comparison of GSC regulators with neural stem cell self-renewal factors identifies common and cell-type-specific self-renewal genes. Importantly, we identify the histone methyltransferase Set1 as a GSC specific self-renewal factor. Loss of Set1 in neural stem cells does not affect cell fate decisions, suggesting a differential requirement of H3K4me3 in different stem cell lineages. Altogether, our study provides a resource that will help to further dissect the networks underlying stem cell self-renewal.en-USResearch Subject Categories::NATURAL SCIENCES::Biology::Organism biology::Developmental biologyJournal Article2021-06-2510.1016/j.devcel.2014.01.020