Zhao, YunlongYou, Siheng SeanZhang, AnqiLee, Jae-HyunHuang, JinlinLieber, Charles2020-01-232019-07-01Zhao, Yunlong, Siheng Sean You, Anqi Zhang, Jae-Hyun Lee, Jinlin Huang, and Charles M. Lieber. 2019. Scalable Ultrasmall Three-dimensional Nanowire Transistor Probes for Intracellular Recording. Nature Nanotechnology 14, no. 8: 783-90.1748-33871748-3395http://nrs.harvard.edu/urn-3:HUL.InstRepos:42241193New tools for intracellular electrophysiology that push the limits of spatiotemporal resolution while reducing invasiveness could provide a deeper understanding of electrogenic cells and their networks in tissues and push progress towards human-machine interfaces. While significant advances have been made in developing nanodevices for intracellular probes, current approaches exhibit a tradeoff between device scalability and recording amplitude. We address this challenge by combining deterministic shape-controlled nanowire transfer with spatially-defined semiconductor-to-metal transformation to realize scalable nanowire field-effect transistor probe arrays with controllable tip geometry and sensor size, which enable recording of up to 100 mV intracellular action potentials from primary neurons. Systematic studies on neurons and cardiomyocytes show that controlling device curvature and sensor size is critical for achieving high amplitude intracellular recordings. In addition, this device design allows for multiplexed recording from single cells and cell networks and could enable future investigations of dynamics in the brain and other tissues.en-USElectrical and Electronic EngineeringGeneral Materials ScienceAtomic and Molecular Physics, and OpticsBioengineeringCondensed Matter PhysicsBiomedical EngineeringJournal Article2020-01-2310.1038/s41565-019-0478-y