Song, LiCohen, DavidOuyang, ZhangyiCao, YangHu, XihaoLiu, Xiaole2022-06-062021-06Song, Li, David Cohen, Zhangyi Ouyang, Yang Cao, Xihao Hu, Xiaole Liu. "TRUST4: Immune Repertoire Reconstruction From Bulk and Single-Cell RNA-Seq Data." Nature Methods 18, no. 6 (2021): 627-630. DOI: 10.1038/s41592-021-01142-21548-70911548-7105https://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37371887We introduce the TRUST4 open-source algorithm for reconstruction of immune receptor repertoires in αβ/γδ T cells and B cells from RNA-sequencing (RNA-seq) data. Compared with competing methods, TRUST4 supports both FASTQ and BAM format and is faster and more sensitive in assembling longer—even full-length—receptor repertoires. TRUST4 can also call repertoire sequences from single-cell RNA-seq (scRNA-seq) data without V(D)J enrichment, and is compatible with both SMART-seq and 5′ 10x Genomics platforms.en-USResearch Subject Categories::TECHNOLOGY::Bioengineering::BioinformaticsResearch Subject Categories::NATURAL SCIENCES::Biology::Cell and molecular biologyJournal Article2022-06-0610.1038/s41592-021-01142-2