Hu, YanhuiRolfs, AndreasBhullar, BhupinderMurthy, Tellamraju S.Zhu, CongBerger, Michael F.Camargo, Anamaria A.Kelley, FontinaMcCarron, SeamusJepson, DanielRichardson, AaronRaphael, JacobMoreira, DonnaTaycher, ElenaZuo, DongmeiMohr, StephanieKane, Michael F.Williamson, JaniceSimpson, AndrewBulyk, Martha L.Harlow, EdwardMarsischky, GeraldKolodner, Richard D.LaBaer, Joshua2019-10-052007Hu, Y., A. Rolfs, B. Bhullar, T. V. S. Murthy, C. Zhu, M. F. Berger, A. A. Camargo, et al. 2007. “Approaching a Complete Repository of Sequence-Verified Protein-Encoding Clones for Saccharomyces Cerevisiae.” Genome Research 17 (4): 536–43. https://doi.org/10.1101/gr.6037607.1088-90511549-5469http://nrs.harvard.edu/urn-3:HUL.InstRepos:41483024The availability of an annotated genome sequence for the yeast Saccharomyces cerevisiae has made possible the proteome-scale study of protein function and protein-protein interactions. These studies rely on availability of cloned open reading frame (ORF) collections that can be used for cell-free or cell-based protein expression. Several yeast ORF collections are available, but their use and data interpretation can be hindered by reliance on now out-of-date annotations, the inflexible presence of N- or C-terminal tags, and/or the unknown presence of mutations introduced during the cloning process. High-throughput biochemical and genetic analyses would benefit from a "gold standard" (fully sequence-verified, high-quality) ORF collection, which allows for high confidence in and reproducibility of experimental results. Here, we describe Yeast FLEXGene, a S. cerevisiae protein-coding clone collection that covers over 5000 predicted protein-coding sequences. The clone set covers 87% of the current S. cerevisiae genome annotation and includes full sequencing of each ORF insert. Availability of this collection makes possible a wide variety of studies from purified proteins to mutation suppression analysis, which should contribute to a global understanding of yeast protein function.en-USJournal Article2019-10-0510.1101/gr.6037607